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Cells from Donors 4-5 were cultured in ImmunoCult ™- XF T Cell Expansion Tool (Brochure # 10981), with (activated) or without (control) ImmunoCult ™ Human CD3/CD28 T Cell Activator (Brochure # 10971) for 48 hours. Supernatants were collected from the cultures, and focus of secreted cytokines were identified using the Human IL-2 ELISA Kit (Catalog # 02006). Activation by either PMA and Ionomycin or ImmunoCult ™ Human CD3/CD28 T Cell Activator brought about raised secretion of IL-2 compared to unstimulated control societies. ( B) Human B cells (Donors) cryopreserved in CryoStor ® CS10 were defrosted and turned on with 1 µg/ mL CD40 and 100 ng/mL IL-21 for 7 days. Supernatants were collected from the cultures and immunoglobulin G (IgG) production was gauged utilizing the Human IgG ELISA Antibody Pair Kit (Magazine # 01994). 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